查看完整版本: C A Mirkin教授纳米技术名篇文章共享

C A Mirkin教授纳米技术名篇文章共享

Nature 382, 607 - 609 (15 August 1996); doi:10.1038/382607a0



[size=5][b]A DNA-based method for rationally assembling nanoparticles into macroscopic materials[/b][/size]


Chad A. Mirkin, Robert L. Letsinger, Robert C. Mucic & James J. Storhoff


Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA


COLLOIDAL particles of metals and semiconductors have potentially useful optical, optoelectronic and material properties1–4 that derive from their small (nanoscopic) size. These properties might lead to applications including chemical sensors, spectro-scopic enhancers, quantum dot and nanostructure fabrication, and microimaging methods2–4. A great deal of control can now be exercised over the chemical composition, size and polydis-persity1,2 of colloidal particles, and many methods have been developed for assembling them into useful aggregates and materials. Here we describe a method for assembling colloidal gold nanoparticles rationally and reversibly into macroscopic aggregates. The method involves attaching to the surfaces of two batches of 13-nm gold particles non-complementary DNA oligo-nucleotides capped with thiol groups, which bind to gold. When we add to the solution an oligonucleotide duplex with 'sticky ends' that are complementary to the two grafted sequences, the nanoparticles self-assemble into aggregates. This assembly process can be reversed by thermal denaturation. This strategy should now make it possible to tailor the optical, electronic and structural properties of the colloidal aggregates by using the specificity of DNA interactions to direct the interactions between particles of different size and composition.



References 1. Schmid, G. (ed.) Clusters and Colloids (VCH, Weinheim, 1994).
2. Hayat, M. A. (ed.) Colloidal Gold: Principles, Methods, and Applications (Academic, San Diego, 1991).
3. Bassell, G. J., Powers, C. M., Taneja, K. L. & Singer, R. H. J. Cell Biol. 126, 863−876 (1994). | Article | PubMed | ISI | ChemPort |
4. Creighton, J. A., Blatchford, C. G. & Albrecht, M. G. J. chem. Soc. Faraday II 75, 790−798 (1979). | ChemPort |
5. Brust, M., Bethell, D., Schiffrin, D. J. & Kiely, C. J. Adv. Mater. 7, 795−797 (1995). | Article | ISI | ChemPort |
6. Dubois, L. H. & Nuzzo, R. G. A. Rev. phys. Chem. 43, 437−463 (1992). | ChemPort |
7. Bain, C. D. & Whitesides, G. M. Angew. Chem. int. Edn. engl. 28, 506−512 (1989). | Article |
8. Shekhtman, E. M., Wasserman, S. A., Cozzarelli, N. R. & Solomon, M. J. New J. Chem. 17, 757−763 (1993). | ChemPort |
9. Shaw, S. Y. & Wang, J. C. Science 260, 533−536 (1993). | PubMed | ISI | ChemPort |
10. Herrlein, M. K., Nelson, J. S. & Letsinger, R. L. J. Am. Chem. Soc. 117, 10151−10152 (1995). | Article | ISI | ChemPort |
11. Chen, J. H. & Seeman, N. C. Nature 350, 631−633 (1991). | Article | PubMed | ISI | ChemPort |
12. Smith, F. W. & Feigon, J. Nature 356, 164−168 (1992). | Article | PubMed | ISI | ChemPort |
13. Wang, K. Y., McCurdy, S., Shea, R. G., Swaminathan, S. & Bolton, P. H. Biochemistry 32, 1899−1904 (1993). | Article | PubMed | ISI | ChemPort |
14. Chen, L. Q., Cai, L., Zhang, X. H. & Rich, A. Biochemistry 33, 13540−13546 (1994). | Article | PubMed | ISI | ChemPort |
15. Marsh, T. C., Vesenka, J. & Henderson, E. Nucleic Acids Res. 23, 696−700 (1995). | PubMed | ChemPort |
16. Mirkin, S. M. & Frankkamenetskii, M. D. A. Rev. Biophys. biomolec. Struct. 23, 541−576 (1994). | ChemPort |
17. Wells, R. D. J. biol. Chem. 263, 1095−1098 (1988). | PubMed | ISI | ChemPort |
18. Wang, Y., Mueller, J. E., Kemper, B. & Seeman, N. C. Biochemistry 30, 5667−5674 (1991). | Article | PubMed | ChemPort |
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[[i] 本帖最后由 rabbithong 于 2007-08-16 14:59 编辑 [/i]]

建议：1）申请一个免费的:box (:hand [url]www.box.net[/url])
2）把分享的文件上传到:box（文件最好加密）
3）这里给出下载连接和密码即可！

谢谢！

搞好链接了,请大家分享
:downloads :box
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不好意思,上一个链接是A. P. Alivisatos教授1996年发表在science的经典文章,同时里面还有很多其他牛人的评述,很有价值的.


Mirkin文章的正确链接是下面的地址:
:downloads :box
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谢谢大家指教,不过我还是不回加密.

纳米棱镜合成的文献见本版:
[url]http://www.nanost.net/bbs/thread-12021-1-1.html[/url]

好文章，多谢楼主分享！！！

[size=6]Mirkin教授发表在sceince上的第一篇生物纳米技术的文章,开辟了金纳米粒子在纳米技术中应用的新领域.[/size]
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[size=5]Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles[/size]
Robert Elghanian, James J. Storhoff, Robert C. Mucic,
Robert L. Letsinger,* Chad A. Mirkin*
A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.

[size=10.5pt] [/size]

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[[i] 本帖最后由 rabbithong 于 2007-08-18 23:36 编辑 [/i]]

:) 很好的介绍！希望以后多多分享！
在这里，贡献永远<收获，贡献越多，收获会更大！:lol

:victory: :victory: :victory: :victory:

[size=4]在上篇文章发表之后,Mirkin小组用金纳米粒子作为生物纳米探针建立了一系列的分析方法检测DNA.[/size]
[size=5]Scanometric DNA Array Detection with Nanoparticle Probes[/size]

T. Andrew Taton,1,2 Chad A. Mirkin,1,2* Robert L. Letsinger1*

A method for analyzing combinatorial DNA arrays using oligonucleotide-modi&THORN;ed gold nanoparticle probes and a conventional &szlig;atbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting pro&THORN;les of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for &szlig;uorophore-labeled targets. In addition, when coupled with a signal ampli&THORN;cation method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous &szlig;uorophore system by two orders of magnitude.


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[[i] 本帖最后由 rabbithong 于 2007-08-17 16:21 编辑 [/i]]

电导检测方法

[size=5]Array-Based Electrical Detection of DNA with Nanoparticle Probes[/size]

So-Jung Park, T. Andrew Taton,* Chad A. Mirkin†

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration&ETH;dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ; 100,000:1.


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[[i] 本帖最后由 rabbithong 于 2007-08-18 23:44 编辑 [/i]]

拉曼检测法,本法开辟了拉曼光谱在生物检测中的新思路.

[size=5]Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection[/size]

YunWei Charles Cao, Rongchao Jin, Chad A. Mirkin*

Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surfaceenhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of grayscale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow-band spectroscopic fingerprint. Six dissimilar DNAtargets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNAtargets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar.

:downloads :link :box
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[[i] 本帖最后由 rabbithong 于 2007-08-18 23:37 编辑 [/i]]

有好东西啊

很好的介绍！希望以后多多分享！

10#发表的DNA分析检测方法结合磁性纳米粒子在分离方面的独特优势,Mirkin小组又发展了高灵敏的新方法检测蛋白质．

[size=5]Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins[/size]
Jwa-Min Nam,* C. Shad Thaxton,* Chad A. Mirkin†
An ultrasensitive method for detecting protein analytes has been developed. The system relies on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate-specific antigen (PSA) in this case] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes. Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe. Because the nanoparticle probe carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and PSA can be detected at 30 attomolar concentration. Alternatively, a polymerase chain reaction on the oligonucleotide bar codes can boost the sensitivity to 3 attomolar. Comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of3 picomdar, six orders of magnitude less sensitive than what is observed with this method.

实在不知道怎么贴图！


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随着研究的深入，DNA片断修饰的金纳米粒子的使用越来越广泛，本文用这种探针用于调控细胞的基因表达，显示了许多独特的优势，详细见下面论文．我的感觉就是别人通过一步步的前行，逐渐得到自己的目的．本研究现在越来越深入到＂生物＂纳米技术中了！

Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation

Nathaniel L. Rosi,* David A. Giljohann,* C. Shad Thaxton, Abigail K. R. Lytton-Jean,
Min Su Han, Chad A. Mirkin†

We describe the use of gold nanoparticle-oligonucleotide complexes as intracellular gene regulation agents for the control of protein expression in cells. These oligonucleotide-modified nanoparticles have affinity constants for complementary nucleic acids that are higher than their unmodified oligonucleotide counterparts, are less susceptible to degradation by nuclease activity, exhibit greater than 99% cellular uptake, can introduce oligonucleotides at a higher effective concentration than conventional transfection agents, and are nontoxic to the cells under the conditions studied. By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, we demonstrated a tunable gene knockdown.

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[size=6]预告！[/size]下面我将刊载本小组在Dip-Pen Nanolithography方面的一系列工作．
Mirkin小组关于本主题发表在其他TOP化学杂志上的工作将视情况作介绍！！！

好东西！这几天正想看！:lol :lol :lol

:lol ，谢谢分享，:victory:

:P 好东西，先下载看看:P

:victory: :victory: