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FUNCTIONALIZATION OF NANOPARTICLES for Biological Applications

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Author: [i]Isaac Koh[/i]z5IE m Ob)F%y5`
Supervisor: Sheryl H. Ehrman, ProfessorsZ(\ZuOjv
University: Department of Chemical and Biomolecular Engineering, University of Maryland
J J3o/i1W Date: 2005
9\ YE&c}:tH4_#c P5w Abstract:[quote][size=4][color=blue]'gw9C1I}+zc9m
Functionalization of metal oxide nanoparticles enables their use in biological applications via hybridization of biological molecules and modification of surface properties. This Ph. D research is aimed at increasing knowledge of the process of metal oxide nanoparticle functionalization for biological applications. The achievements presented in this dissertation can be divided into three categories: i) a fluorescence-based quantitative evaluation of surface coverage and bio-activity of antibodies immobilized on magnetic nanoparticles (MNPs), ii) differential functionalization of SiO2/TiO2 mixed nanoparticles via preferential binding of phosphonic acids to TiO2 and subsequent trimethyl silyl group binding to the remaining surface, and iii) X-ray scattering (XRS)-mediated detection of peak shifts of a biological substrate, Escherichia coli (E. coli), as a function of applied magnetic field strength and magnetic nanoparticle concentration in a cell growth medium. In a study of MNP surface modification, quantitative evaluation of anti-mouse IgG binding on MNPs and bioactivity on MNPs was conducted via fluorescence assays. Nanosize -Fe2O3 particles were hybridized with anti-mouse IgG via silane chemistry with 3-aminopropyltriethoxy silane and glutaraldehyde activation. A chemisorption isotherm via fluorescence assays demonstrated that immobilization of anti-mouse IgG can be stoichiometrically controlled with the surface coverage at saturation corresponding to 36% of the theoretical limit. The immobilized anti-IgG retains ~50% of its bioactivity at saturation. Differential functionalization of SiO2/TiO2 mixed nanoparticles was demonstrated via aqueous-phase preferential binding of phosphonic acids to TiO2 and subsequent binding of trimethyl silyl group to the remaining surface. SiO2/TiO2 mixed nanoparticles with three different mole ratios of Si/Ti together with pure SiO2 and TiO2 nanoparticles were used in comparative XPS study of differential functionalization. Differential functionalization of metal oxide-metal oxide mixed nanoparticles demonstrated herein adds a route to multifunctional nanoparticles. An In situ XRS study of E. coli in applied magnetic fields up to 423 mT was performed. Two peaks, a sharp peak at q = 0.528 Å-1 (1.189 nm) and a diffuse peak at q = 0.612 Å-1 (1.027 nm), were detected in XRS of MNP-absent E. coli culture. The presence of SiO2/-Fe2O3 MNPs at 40 mg/L in E. coli growth medium changes the sharp peak to the lower side of q as a function of applied magnetic field strength, while the position of the diffuse peak is invariable. 362 mT was found to be a critical magnetic field strength, at which the sharp peak disappears. This study demonstrates magnetic field-assisted interactions between E. coli cell membranes and MNPs.[/color][/size][/quote]q&M&y(GyI
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[[i] 本帖最后由 nano 于 2006-11-28 18:55 编辑 [/i]]

FUNCTIONALIZATION OF NANOPARTICLES for Biological Applications

很好，谢谢！:P :)

[quote]原帖由 [i]panchao[/i] 于 2007-02-17 08:49 发表
q t5`A"]E$txc:\ 很好，谢谢！:P :) [/quote] w e Jb,iR ~
哈哈，楼主把我的话抢了。但我确实不明白，而且还迷盲

:victory: :victory: :victory:

回复 #1 panchao 的帖子

hao!!!!!!!!!!!!!

回复 #1 nano 的帖子

hao!!!!!!!!!!!

回复 #4 tooold 的帖子

把帖子跟错地方了，在这里
8{PX;f9L)~h:PO [url]http://www.nanost.net/bbs/viewthread.php?tid=1232&highlight=Functionalization%2Bof%2BNanoparticles%2Bfor%2BB[/url]

非常好的书，非常感谢

FUNCTIONALIZATION OF NANOPARTICLES for Biological Applications
]bbh"Wlusx$P C7eeI_"_
很好，谢谢！

:victory: :victory: :victory:

thanks for share. :victory:

;P ，有国语的就更好了

:victory: :victory:

支持楼主，将来我可能就要做纳米生物了，多谢！！！

:handshake :victory:

回复 1# 的帖子

好书，多谢！:lol

:handshake :handshake

不错啊 ，看看啊 ，好东西啊

good

Have a look,thanks for your share

怎么看不到啊

怎么看不到链接啊 ，在看看